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. 2023 May 4;18(5):e0284964. doi: 10.1371/journal.pone.0284964

Fig 5. ZIKV burden in fetal tissues and fluids.

Fig 5

A) Amniotic fluid ZIKV RNA levels determined by RT-qPCR (left panel) and infectious ZIKV determined by plaque assay (right panel). Limit of detection for the RT-qPCR fluid assay shown on the left is 150 copies/mL, this is represented by the dashed line. On the right, the dashed line represents the limit of detection of 0.7 PFU/mL for the plaque assay. Four amniotic fluid samples were analyzed by plaque assay based on positive detection of vRNA by RT-qPCR. B) ZIKV RNA isolated from fetal tissues and fluids; viral loads are measured in vRNA copies/mL of fluid (CSF) or vRNA copies/mg of tissue. Limit of detection for the RT-qPCR fluid assay (CSF) is 150 copies/mL and the theoretical limit of detection for the RT-qPCR assay for the tissues is 3 copies/mg. Only samples above the limit of detection are shown.