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. 2023 May 4;18(5):e0285012. doi: 10.1371/journal.pone.0285012

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© 2023 C. M. et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 4 — Ocimum tenuiflorum extract at different non-cytotoxic concentrations was tested in the forskolin-induced cortisol release assay using human adreno-carcinoma (NCI-H295R) cells. The cortisol released by the cells was analyzed using a cortisol-HTRF kit, and the percentage of cortisol inhibited by the test substance is shown. The higher the percentage of cortisol inhibited, the better the activity.