Table 1.
Antibodies used:
| Antigen | Clone | Isotype | Fluorochrome | Source of primary Ab | Details of secondary labelling | |
|---|---|---|---|---|---|---|
| Tetramer staining | ||||||
| CD3ϵ | PPT3 | Mouse IgG1 | APC | Southern Biotech | ||
| CD8β | PPT23 | Mouse IgG1 | PE | Bio-Rad | ||
| CD4 | 74-12-4 | Mouse IgG2b | PerCP-Cy5.5 | BD Biosciences | ||
| CD45RA | MIL13 | Mouse IgG1 | FITC | Bio-Rad | ||
| CCR7 (CD197) | 3D12 | Rat IgG2a | BV711 | BD Biosciences | ||
| Intracellular cytokine staining | ||||||
| CD8β | PPT23 | Mouse IgG1 | FITC | Bio-Rad | ||
| CD4 | 74-12-4 | Mouse IgG2b | PerCP-Cy5.5 | Bio-Rad | ||
| IFNγ | P2G10 | Mouse IgG1 | AF647 | BD Biosciences | ||
| TNFα | Mab11 | Mouse IgG1 | BV421 | BioLegend | ||
| IL-2 | A150D3F1 | Mouse IgG2a | PE-Cy7 | Invitrogen | Anti-mouse IgG2a, clone RMG2a-62, BioLegend | |
| B cell staining with HA-probe | ||||||
| CD79α | HM47 | Mouse IgG1 | PerCP-Cy5.5 | BioLegend | ||
| Blimp1 | 3H2E8 | Mouse IgG1 | AF647 | Santa Cruz Biotechnology | ||
| IRF4 | 3E4 | Rat IgG1 | PE | ThermoFisher | ||
| Bcl-6 | K112-91 | Mouse IgG1 | BV421 | BD Biosciences | ||
| Pax5 | 1H9 | Rat IgG2a | BV510 | BD Biosciences | ||
| Tfh staining | ||||||
| CD3ϵ | BB23-8E6-8C8 | Mouse IgG2a | PerCP-Cy5.5 | BD Biosciences | ||
| CD4 | 74-12-4 | Mouse IgG2b | AF488 | Southern Biotech | Goat anti-mouse IgG2b, Jackson | |
| ICOS (CD278) | C398.4A | Hamster IgG | BV605 | BioLegend | ||
| Bcl-6 | K112-91 | Mouse IgG1 | BV421 | BD Biosciences | ||