Table 1. Small guide RNA (gRNA) sequence for PLS3 gene knockout.
| No. | Targeting site | Name | Sequence (5’–3’) |
|---|---|---|---|
| 1 | ATGTATTCTGGCGCCATCT TGG | PLS3-gRNA-UP1 | TAGGatgtattctggcgccatct |
| PLS3-gRNA-DOWN1 | aaacAGATGGCGCCAGAATACAT | ||
| 2 | TACATACATACATAGATGA TGG | PLS3-gRNA-UP2 | TAGGtacatacatacatagatga |
| PLS3-gRNA-DOWN2 | aaacTCATCTATGTATGTATGTA | ||
| 3 | TCTCAGGTGAAGTGCACA TGG | PLS3-gRNA-UP3 | TAGGtctcaggtgaagtgcaca |
| PLS3-gRNA-down3 | aaacTGTGCACTTCACCTGAGA | ||
| PLS3-gRNA-UP4 | TAGGATCCAGGTCTACAGGAAAG | ||
| PLS3-gRNA-down4 | aaacctttcctgtagacctggat | ||
| 4 | CCT CTTTCCTGTAGACCTGGAT | PLS3-gRNA-UP5 | TAGGATCCAGGTCTACAGGAAAG |
| PLS3-gRNA-down5 | AAACCTTTCCTGTAGACCTGGAT |
The gRNAs were designed based on Rattus norvegicus (Norway rat) genome assembly Rnor_6.0 (rn6), using CRISPR Design Tool (http://tools.genome-engineering.org).
Gene ID: 81748; Location: Chromosome X - NC_005120.4.
The words in red represented the protospacer adjacent motif (PAM) sequences (5’–3’).