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| Construct | kcat (min–1) | KM (µM) | kcat/KM (µM–1 min–1) |
|---|---|---|---|
| AncD1D2, no dsRNA | 1117±94.5 | 35812±6,367 | 0.031 |
| AncD1D2, BLT dsRNA | 147.8±6.3 | 256±67.5 | 0.577 |
| AncD1DEUT, no dsRNA | 144.1±14.9 | 2550±855 | 0.055 |
| AncD1DEUT, BLT dsRNA | 40.31±3.78 | 336.4±142 | 0.12 |
| AncD1VERT, no dsRNA | - | - | - |
| AncD1VERT.7, no dsRNA | 257.7±28.7 | 61739±12,886 | 0.004 |
| AncD1VERT.7, BLT dsRNA | 24.87±3.52 | 5173±1929 | 0.005 |
