Extended Data Fig. 8. Differential transcriptomics of differentiated urothelia originating from convalescent mice recapitulate in vivo studies, related to Fig. 4.
RNA-seq data of UTI89 infected and mock-infected differentiated urothelia (Fig. 4c) was used to perform differential analysis. (A) Volcano plots comparing UPEC infected vs mock-infected Naïve, Resolved, and Sensitized differentiated urothelia (UPEC infection response). (B) Differential gene expression of Ptgs2 between Naïve, Resolved, and Sensitized differentiated urothelia with or without UTI89 infection is visualized as a heatmap. (C) A volcano plot comparing UTI89 infected Sensitized vs Resolved differentiated urothelia was performed. (D) Pathway analysis was used to assess the biological pathways enriched in differentially expressed genes in UPEC infected Sensitized differentiated urothelia relative to Resolved differentiated urothelia, and significance was determined by a right-tailed Fisher’s exact test, with P-adjusted <0.05 considered significantly enriched pathways. Shown are selected pathways with z-score > 2 and –log(p-value) > 2 from the specific enriched pathways by IPA.