Global methylation changes associated with transformation and recapitulated in lung tumors. A. Number of methylated genes across HBEC2, HBEC4, and HBEC26 transformed by Jamestown (JT), Blue Pucker (BP), Unflavored (UF) or the KRC. B. Number of methylated genes with reduced expression from HBEC2, HBEC4, and HBEC26 transformed clones that are also epigenetically silenced in lung tumors. C. Venn diagram showing common and distinct genes that are methylated in transformed clones from cigarette (KRC) and e-liquids and also methylated in lung tumors. D. Significantly altered canonical pathways comprising genes methylated in cigarette transformed clones and lung tumors. E. Significantly altered canonical pathways comprising genes methylated in e-liquid transformed clones and lung tumors. F. Number of genes with reduced expression from HBEC2, HBEC4, and HBEC26 transformed clones that become methylated in lung tumors. Abbreviation: Sig, signaling