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. Author manuscript; available in PMC: 2024 May 2.
Published in final edited form as: Cell Metab. 2023 Apr 20;35(5):855–874.e5. doi: 10.1016/j.cmet.2023.03.022

Figure 2. S1P is synthesized in glia and transported to neurons.

Figure 2.

(A) Scheme of the structure of the CDaseT2A and SK1T2A alleles. (B) Both CDase and SK1 are expressed in glia of the adult CNS. Expression of CDaseT2A>nls-mCherry (red) colocalized with anti-Repo (green), marking the glia nuclei in adult CNS (Top). Expression of SK1T2A>nls-mCherry (red) colocalized with anti-Repo (green) (Bottom). Scale bar: 5 μm. (C) Increased synthesis of S1P results in severe climbing defects on Day 10 at 29°C degree (n>5). (D) Expression of ELOVL1 in glia increases the level of S1P in neurons. mCD8-GFP (green) labels the glial membrane, and anti-S1P (red) documents the expression pattern of S1P in the adult CNS. White arrows indicate abnormal glial membranes caused by glial ELOVL1 expression. The white dotted box indicates the region that is enlarged. Scale bar: 5 μm