KEY RESOURCE TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| anti-elav | DSHB Cat# 7E8A10 | RRID:AB_528218 |
| anti-Repo | DSHB Cat# 8D12 | RRID:AB_528448 |
| Anti-Hemese | 121 | N/A |
| anti-mouse-647 | Jackson ImmunoResearch Labs Cat# 715-605-151 | RRID:AB_2340863 |
| anti-rabbit-Cy3 | Jackson ImmunoResearch Labs Cat# 712-165-153 | RRID:AB_2340667 |
| anti-GFP | Abcam Cat# ab6662 | RRID:AB_305635 |
| anti-HA | BioLegend Cat# 902301 | RRID:AB_2565018 |
| anti-actin | Millipore Cat# MAB1501 | RRID:AB_2223041 |
| anti-Sphingosine 1-phosphate | Echelon Biosciences Cat# Z-P300 | |
| anti-Relish | DSHB Cat# 21F3 | RRID:AB_1553772 |
| anti-RFP | Invitrogen Cat# R10367 | RRID:AB_10563941 |
| anti-Draper | DSHB Cat# 8A1 | RRID:AB_2618106 |
| anti-Neurofilament-H | BioLegend Cat# 835601 | RRID:AB_2565349 |
| anti-Iba-1 | FUJIFILM Wako Shibayagi Cat# 013-26471 | RRID:AB_2687911 |
| anti-ACOX1 | Sigma-Aldrich Cat# HPA021195 | RRID:AB_1844528 |
| anti-CD8 | Abcam Cat# ab236230 | RRID:AB_2756381 |
| anti-GFAP | Agilent Cat# N1506 | RRID:AB_10013482 |
| Bacterial and Virus Strains | ||
| NEB® 5-alpha Competent E. coli | NEB | Cat#C2987 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| CHAPS hydrate | Sigma-Aldrich | Cat# C9426 |
| cOmplete™ Protease Inhibitor Cocktail | Roche | Cat# 11697498001 |
| PhosSTOP™ | Sigma-Aldrich | Cat# 4906845001 |
| 10x Tris/Glycine Buffer | Biorad | Cat# 1610734 |
| Bezafibrate | Sigma-Aldrich | Cat# BP755 |
| N-acetylcystein amide | Sigma-Aldrich | Cat# A0737 |
| 4%−15% Mini-PROTEAN TGX precast native gel | Biorad | Cat# 4561086 |
| Fingolimod (FTY720) | Millipore sigma | Cat# 162359-56-0 |
| Experimental Models: Organisms/Strains | ||
| UAS-2xEGFP, hs-Cre,vas-dϕC31 | 122 | N/A |
| Repo-Gal4 | BDSC_7415 | RRID: BDSC_7415 |
| Hml-Delta-Gal4 | 88 | N/A |
| UAS-dELOVL RNAi | BDSC_50710 | RRID: BDSC_50710 |
| UAS-hELOVL1 | BDSC_77932 | RRID: BDSC_77932 |
| dACOX1 T2A | This paper | N/A |
| UAS-nls. mCherry | BDSC_38424 | RRID: BDSC_38424 |
| UAS-lacZ | Bellen lab stock | N/A |
| UAS-Draper | BDSC_67035 | RRID: BDSC_67035 |
| UAS-Draper RNAi | BDSC_67034 | RRID: BDSC_67034 |
| UAS-Luciferase RNAi | BDSC_35785 | RRID: BDSC_35785 |
| UAS-CDase RNAi | BDSC_36764 | RRID: BDSC_36764 |
| UAS-SK1 RNAi | BDSC_36747 | RRID: BDSC_36747 |
| UAS-SK2 RNAi | BDSC_35570 | RRID: BDSC_35570 |
| UAS-CDase | 123 | N/A |
| UAS-SK1 | 123 | N/A |
| UAS-mCD8GFP | BDSC_32184 | RRID: BDSC_32184 |
| Repo-LexA-GAD | BDSC_67096 | RRID: BDSC_67096 |
| LexAop-ELOVL1 | This paper | N/A |
| Sply RNAi | VDRC_105752 | RRID: FlyBase_FBst0324722 |
| UAS-sply | This paper | N/A |
| UAS-SGPL1 | This paper | N/A |
| UAS-Relish RNAi | BDSC_33661 | RRID: BDSC_33661 |
| dACOX1ywg | N/A | |
| BcF6-mCherry | 124 | N/A |
| P{w[+mC]=srpHemo-Moe.3XmCherry}3 | 125 | N/A |
| UAS-lace | DGGR_109638 | RRID: DGGR_109638 |
| UAS-spt | 126 | N/A |
| Tubulin Gal80 | 86 | N/A |
| Perineurial glia Gal80 | 86 | N/A |
| TubGal80ts;Repo Gal4 | S. Schirmeir & C. Klambt | N/A |
| Software and Algorithms | ||
| ImageJ | Version 2.0 | https://www.imagej.nih.gov |
| Prism7 | Graph Pad | https://www.graphpad.com |
| Zen Blue | Zeiss | https://www.zeiss.com |
| Zen Black | Zeiss | https://www.zeiss.com |
| Snapgene | Snapgene | www.snapgene.com |
| Imagelab | Bio-rad | https://www.bio-rad.com/en-us/product/image-lab-software |
| Other | ||