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. 2023 May 4;14:2586. doi: 10.1038/s41467-023-38186-1

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Three-dimensional tSNE plots showing all astrocyte nuclei color-coded by astrocyte state (Ast1—protoplasmic astrocytes, Ast2—reactive astrocytes with misexpression of non-astrocyte lineage genes, and Ast3—reactive astrocytes with expression of inflammatory genes. b Three-dimensional tSNE plots showing all astrocyte nuclei color-coded by disease condition. c Gene expression dot plots showing select gene marker expression for the astrocyte states. d tSNE plots showing the enrichment of gene signatures used for astrocyte clustering in astrocyte nuclei.