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. 2023 May 5;14(5):306. doi: 10.1038/s41419-023-05806-z

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Representative live cell confocal fluorescence microscopy images showing spheroid morphology and cell distribution on day 4 post-seeding. HCT116 cells were stained with cell tracker CMFDA (green), SW620 and MDST8 cells were stained with CMRA (red) fluorescent probes, LoVo cells express GFP. Scale bars = 100 μm. b Cell growth in cocultured 3D spheroids. Cell growth was measured in cocultured tumor spheroids by means of image analysis quantifying the fluorescence intensity of cell trackers that represent the cell number, and normalized to the monoculture as a control. The bars represent the average of cell growth and the error bars represent the standard deviation (n = 3). Statistical significance was calculated using a one-way ANOVA followed by Student’s t-test. P-values of <0.05 and 0.01 were considered significant (*) and highly significant (**), respectively.