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. 2023 May 5;14(5):306. doi: 10.1038/s41419-023-05806-z

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Representative widefield fluorescence microscopy images showing MDST8 migration and invasion through transwell membrane with and without Matrigel coating after 2 day exposure to HCT116 or LoVo in the bottom wells. MDST8 exposed to only media without cells were taken as a control. Cells were treated with either DMSO vehicle or 2.5 μM of 5-FU. Cell nuclei were stained with Hoechst (blue). Scale bars = 100 μm. b Invasion rate of MDST8 presented as the percentage of cell invasion through Matrigel-coated transwell membrane relative to the cell migration through the non-Matrigel coated transwell membrane. The bars represent the average and the error bars represent the standard deviation (n = 3). Statistical significance was calculated using a one-way ANOVA followed by Student’s t-test. P-values of <0.05 and 0.01 considered significant (*) and highly significant (**), respectively, when compared to the control.