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. 2023 May 5;14(5):306. doi: 10.1038/s41419-023-05806-z

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — CMS4 cells were treated with either metabolite_vehicle or metabolite_5-FU of a HCT116 or b LoVo CMS1 cells, and were exposed to different concentrations of 5-FU for 3 days. Cells treated with media only were taken as a control. Cell viability was measured using MTS assays. The squares, circles, and triangles represent the average viability normalized to the control and the error bars represent the standard deviation (n = 3). Statistical significance was calculated using a one-way ANOVA followed by Student’s t-test. P-values of <0.05 and 0.01 were considered significant (*) and highly significant (**), respectively, when compared to the control.