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miR-486-5p targets CEMIP
(A) TargetScan predicted the binding sites between miR-486-5p and CEMIP. (B) Dual luciferase reporter assay was used to identify the interaction between miR-486-5p and CEMIP. (C) qPCR showed that miR-486-5p mimic inhibited CEMIP expression in hypoxic PASMCs. (D,E) Western blot analysis results verified that the miR-486-5p inhibitor upregulated CEMIP protein level, but the miR-486-5p mimic downregulated CEMIP protein level. * P<0.05, ** P<0.01.
