TABLE 1.
Strains and plasmids used in the study
| Strain or plasmid | Relevant genotype or phenotype | Source or reference |
|---|---|---|
| Strains | ||
| S. marcescens Rio-5 | Clinical strain harboring the natural plasmid pRio-5 (Rio de Janeiro, Brazil, 1997) | This study |
| E. coli DH5α | supE44 ΔlacU169 (φ80 lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | 44 |
| Plasmids | ||
| pRio-5 | Natural plasmid from S. marcescens Rio-5 containing blaBES-1 gene; resistance phenotype: ESBL | This study |
| pClRio-5 | Recombinant plasmid containing a 2.2-kb fragment with blaBES-1; resistance phenotype: ESBL, kanamycin | This study |
| pClRio-2 | Recombinant plasmid containing blaCTX-M-8; resistance phenotype: ESBL, kanamycin | 7 |
| pBK-CMV | Phagemid vector; resistance phenotype: kanamycin | Stratagene |