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. 2023 Apr 21;14:1182963. doi: 10.3389/fimmu.2023.1182963

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Copyright © 2023 Kubinski, Beicht, Zdora, Biermann, Puff, Gerlach, Tscherne, Baumgärtner, Osterhaus, Sutter, Prajeeth and Rimmelzwaan

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Quantification of TBEV RNA copies in the periphery, CNS, and GIT of vaccinated and infected mice. Cleared organ homogenates collected on the day of sacrifice were tested for the presence of TBEV RNA by real-time RT-qPCR. Mice were either sacrificed at (A) 8 dpi or (B) stayed in the experiment until the study endpoint (16 dpi). The median values of the respective treatment groups are indicated in the graphs. Mice were immunized with PBS (gray circle), MVA (non-filled triangle), FSME-IMMUN^® (non-filled blue circle), or MVA-prME (red triangle).