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. 2023 Apr 21;14:1182963. doi: 10.3389/fimmu.2023.1182963

Figure 6.

Figure 6

Histological and immunohistochemical analyses of the jejunum at 8 dpi. (A–C) H&E-stained sections of the jejunum of TBEV-infected mice which were either treated with PBS (A) or vaccinated with FSME-IMMUN® (B) or MVA-prME (C). (A) Marked hypercellularity/inflammatory cell infiltrates in the submucosal plexus (asterisk) and, to a lesser extent, in the myenteric plexus (arrowhead) as well as cellular necrosis with karyorrhectic, karyolytic, and pyknotic cells (arrowhead) are present. (B, C) No significant microscopic lesions within the submucosal (asterisk) and myenteric plexus (arrowhead) are present in vaccinated mice. (D–F) IHC for TBEV E antigen of the jejunum of TBEV-infected mice which were either treated with PBS (D) or vaccinated with FSME-IMMUN® (E) or MVA-prME (F). (D) Immunohistochemically, TBEV-positive neurons are observed in the myenteric plexus (arrowhead) but not in the submucosal plexus (asterisk). (E, F) No immunopositive cells are present in the submucosal (asterisk) or myenteric plexus (arrowhead). Band-like, not cell-associated, brownish discoloration in (E) represents a staining artifact due to the unspecific binding of the antibody to serum components. Scale bars: 20 µM.