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. 2023 May 5;4(2):102326. doi: 10.1016/j.xpro.2023.102326

Figure 1.

Figure 1

pGTM_CoV2_NSP5_004_SUMO construct design

(A) Multiple cloning site of the original vector pET15_SUMO2_NESG showing BsaI restriction sites used for cloning the CDS of 3CLpro. Maps are generated using SnapGene® software (from Insightful Science; available at snapgene.com). The synthetic gene for 3CLpro was obtained from Genscript, Inc. with standard Genscript codon optimization for E. coli expression.

(B) Final expression vector pGTM_CoV2_NSP5_004_SUMO.

(C) Schematic representation of the expressed His6-SUMO-3CLpro.