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Immobilized metal (nickel) affinity chromatography (IMAC)
(A) Representative chromatogram showing the entire process of protein loading, flow through collection, and protein elution.
(B) SDS – PAGE showing total lysate (TL), soluble extract (S), flowthrough (FT) and fractions eluted in peak 1 (P1) of the IMAC. His6-SUMO-3CLpro (molar mass of ca. 46 kDa) is detected in lanes corresponding to peak P1.
