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Materials preparation and data processing for IC50 determination
(A) Schematic of 96-well plate set-up for dose response assay to determine IC50.
(B) Table of reagents for each well (final DMSO concentration = 4 %).
(C) Representative experimental raw data showing the increase of fluorescence intensity as a function of reaction time.
(D) Representative dose response curve for Narlaprevir, error bars represent SD's of triplicate measurements.
