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Experimental set up for the crystallization of SARS-CoV-2 3CLpro by hanging drop and crystal recovery and storage
(A and B) (A) Preparation of the crystallization plate, and (B) crystallization drops deposition.
(C and D) (C) 3CLpro typically grows as bundles of sword-shaped crystals which (D) are fished using cryo-loops, flash-cooled in liquid nitrogen for storage, and shipped to the synchrotron facility for data collection.
