Table 1.
Preparation of the crystallization plate
[Ammonium acetate at pH 7.0] (mM) | ||||||
---|---|---|---|---|---|---|
P E G 4 0 0 0 (%) |
20 | 30 | 40 | 50 | 60 | |
20 | AMA 1 M = 10 μL | AMA 1 M = 15 μL | AMA 1 M = 20 μL | AMA 1 M = 25 μL | AMA 1 M = 30 μL | |
PEG 50 % = 200 μL | PEG 50 % = 200 μL | PEG 50 % = 200 μL | PEG 50 % = 200 μL | PEG 50 % = 200 μL | ||
H2O = 290 μL | H2O = 285 μL | H2O = 280 μL | H2O = 275 μL | H2O = 270 μL | ||
25 | AMA 1 M = 10 μL | AMA 1 M = 15 μL | AMA 1 M = 20 μL | AMA 1 M = 25 μL | AMA 1 M = 30 μL | |
PEG 50 % = 250 μL | PEG 50 % = 250 μL | PEG 50 % = 250 μL | PEG 50 % = 250 μL | PEG 50 % = 250 μL | ||
H2O = 240 μL | H2O = 235 μL | H2O = 230 μL | H2O = 225 μL | H2O = 220 μL | ||
30 | AMA 1 M = 10 μL | AMA 1 M = 15 μL | AMA 1 M = 20 μL | AMA 1 M = 25 μL | AMA 1 M = 30 μL | |
PEG 50 % = 300 μL | PEG 50 % = 300 μL | PEG 50 % = 300 μL | PEG 50 % = 300 μL | PEG 50 % = 300 μL | ||
H2O = 190 μL | H2O = 185 μL | H2O = 180 μL | H2O = 175 μL | H2O = 170 μL |