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Loading / Washing Buffer at pH 8.0 (for immobilized metal affinity chromatography, IMAC)
| Reagent | Final concentration | Amount for 1 L |
|---|---|---|
| Tris-HCl | 20 mM | 2.42 g |
| NaCl | 300 mM | 17.53 g |
| Imidazole | 10 mM | 0.68 g |
| Dithiothreitol (DTT) 1 M | 1 mM | 1 mL |
Prepare 1 L, adjust to pH 8.0, and store at room temperature (20–25 °C) for up to three days. Pass through 0.22 μm filter and degas before use
