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Elution buffer at pH 8.0 (for IMAC)
| Reagent | Final concentration | Amount for 0.5 L |
|---|---|---|
| Tris-HCl | 20 mM | 1.21 g |
| NaCl | 300 mM | 8.76 g |
| Imidazole | 300 mM | 10.21 g |
| DTT 1 M | 1 mM | 0.5 mL |
Prepare 0.5 L, adjust to pH 8.0, and store at room temperature (20–25 °C) for up to three days. Pass through 0.2 μm filter and degas before use
