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Size exclusion chromatography (SEC) buffer at pH 7.5
| Reagent | Final concentration | Amount for 0.5 L |
|---|---|---|
| Tris-HCl | 20 mM | 1.21 g |
| NaCl | 50 mM | 1.46 g |
| EDTA | 1 mM | 0.146 g |
| TCEP 0.1 M | 1 mM | 5 mL |
Prepare 0.5 L, adjust to pH 7.5, and store at 4 °C for up to one week. Pass through 0.2 μm filter and degas before use
