Förster resonance energy transfer (FRET) buffer at pH 7.3

Reagent	Final concentration	Amount for 0.1 L
Tris-HCl	20 mM	0.242 g
NaCl	100 mM	0.292 g
EDTA	1 mM	0.0292 g
DTT 1 M	1 mM	1 mL
BSA∗	0.1 mg mL−1

Prepare 0.1 L, adjust to pH 7.3, and store at 4 °C

∗To be added just before setting up the assay