TABLE 1.

Bacterial strains and plasmids used in this work

Strain or plasmid	Descriptiona	Source or reference
Strains
S. maltophilia
D457 strain	Bronchial-aspiration isolate	Laboratory collection 2
D457R strain	D457 derivative strain with the MDR phenotype that overproduces Omp54	Laboratory collection 2
E. coli
KZM120	ΔacrAB::Tn903 Kanr	Dzwokai Ma 29
AA68	KZM120 Rfr derivative, selected on 50 mg of rifampin/ml	This work
LE392	supE44 supF58 hsdR514 galK2 galT22 metB1 trpR55 lacY1	Laboratory collection 43
AA81	LE392 derivative, ΔacrAB::Tn903 Kanr	This work
TG1	supE hsdΔ5 thi Δ(lac-proAB) F′[traD36 proAB+ lacIqlacZΔM15]	Laboratory collection 43
AA72	TG1 derivative, ΔacrAB::Tn903 Kanr	This work
HB101	supE44 hsdS20 (rB mB) recA13 ara-14 proA2 lacY1 galK2 rpsL20 xyl-5 mtl-1	Laboratory collection 43
Plasmids
pLAFR3	Low-copy-number cloning cosmid, Tcr	Fernando Rojo 45
pAS1	pLAFR3 derivative that contains a 30-kbp BamHI DNA fragment from S. maltophilia D457R strain	This work
pUC19	High-copy-number cloning vector, Apr	Laboratory collection 43
pCK01	Low-copy-number cloning vector, Cmr	Amparo Haro 13
pAS2	pCK01 derivative that contains smeDEF on the 9-kb digestion fragment E2	This work
pRK2013	KanroriV ColE1 RK2-mob+ RK2-tra+, helper plasmid in triparental matings	Laboratory collection 14

^aKan^r, kanamycin resistance; Rf^r, rifampin resistance; Tc^r, tetracycline resistance; Ap^r, ampicillin resistance; Cm^r, chloramphenicol resistance.