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. 2023 Apr 21;4(2):102177. doi: 10.1016/j.xpro.2023.102177

Figure 6.

Figure 6

Changes in stem cell markers in BON cells in co-culture with HMEC-1 cells and CAFs at different ratios

(A) Representative immunofluorescence images of 3D ring assays containing differing ratios of HMEC-1 to CAFs to BON cells. Colors are shown in legends to the right of the images. Scale bars = 250 μm. Images are 100 μm z-stacks projected for maximum focus through the Extended Depth of Field algorithm. These images are then processed with the Analyze Particles measurement algorithm in FIJI to determine total positive area for each stain.

(B) Quantification of positive area for each stain relative to DAPI area. The results are from triplicate experiments. No statistical significance was observed for different ratios; however, there are significantly higher levels of CD36 compared to ALDH1A1 for all samples (ANOVA, p < 0.05). Data shown are averages + SEM for triplicate samples. See also Figure S1 and Table S2.