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Master mix for RT-qPCR reaction
| Reagents | Final concentration | Volume (μL) |
|---|---|---|
| Bright Green 2× qPCR MasterMix | 1× | 10 |
| Forward Primer (10 μM) | 300 nM | 0.6 |
| Reverse Primer (10 μM) | 300 nM | 0.6 |
| cDNA | Variable | Variable |
| Nuclease-free Water | Variable | Variable |
| Total | N/A | 20 |
