Fig. 2.

Behavioral profiles of zebrafish by the long-term exposure to acrylamide (AA) in the light/dark preference test. (A) Schematic of the zebrafish study by the long-term exposure to acrylamide. (B and C) Effects of long-term exposure to acrylamide for 180 d on body mass and length of zebrafish, respectively. (D) Representative swimming trajectories of zebrafish in the control group and three acrylamide exposure groups (0 mM wide type, 0.125 mM, 0.25 mM, and 0.5 mM). The left side is the light chamber, and the right side is the dark chamber. (E) Radar chart of 12 behavioral parameters of zebrafish in different groups (0 mM wide type, 0.125 mM, 0.25 mM, and 0.5 mM). a, duration; b, distance; c, average velocity (cm/s); d, accelerated speed; e, average entry time duration (s); f, turning angle (°); g, turning angle (°)/time; h, activity; i, rapid move ratio; j, normal move ratio; k, freezing time ratio (s); l, freezing time duration (s). (F) Heatmap visualization of zebrafish trajectories in the light/dark preference test. (G) Duration time spent in the light or dark zone of total time (%). (H) Distance traveled in the light or dark zone of total distance (%). (I) Freezing duration time (s) in the light or dark zone. (J) Traversing times between the light and dark zones. (K) Hierarchical clustering of zebrafish in the light/dark preference test. All the histograms were present with mean ± SEM, while all behavioral parameter data were analyzed by the two-way ANOVA followed by multiple comparisons. The level of significance was defined as *P < 0.05, **P < 0.01, ****P < 0.0001; ^#P < 0.05, ^##P < 0.01, ^###P < 0.001, ^####P < 0.0001 (* indicates significance between different groups and # indicates significance between different regions within the same group).