Fig. 3.

Behavioral profiles of zebrafish by the long-term exposure to acrylamide in the novel object exploration test and the social preference test. (A) Representative swimming trajectories of zebrafish in the control group and three acrylamide exposure groups (0 mM wide type, 0.125 mM, 0.25 mM, and 0.5 mM). A novel object for zebrafish was placed in the left part (Zone 1) and the right part was Zone 2. (B) Heatmap visualization of zebrafish trajectories in the novel object exploration test. (C) Duration time spent in Zone 1 or Zone 2 of total time (%). (D) Distance traveled in Zone 1 or Zone 2 of total distance (%). (E) Representative swimming trajectories of zebrafish in different groups (0 mM wide type, 0.125 mM, 0.25 mM, and 0.5 mM). (F) Radar chart of 12 behavioral parameters of zebrafish in different groups (0 mM wide type, 0.125 mM, 0.25 mM, and 0.5 mM). a, duration; b, distance; c, average velocity (cm/s); d, accelerated speed; e, average entry time duration (s); f, turning angle (°); g, turning angle (°)/time; h, activity; i, rapid move ratio; j, normal move ratio; k, freezing time ratio (s); l, freezing time duration (s). (G) Heatmap visualization of zebrafish trajectories in the social preference test. (H) Duration time spent in the left or right chamber of total time (%). (I) Distance traveled in the left or right chamber of total distance (%). (J) Traversing times between the left and right chambers. (K) Numbers of crossing the middle line. (L) Hierarchical clustering of zebrafish in the social preference test. All the histograms were present with mean ± SEM, while all behavioral parameter data were analyzed by the two-way ANOVA followed by multiple comparisons or the one-way ANOVA followed by the Turkey post hoc test. The level of significance was defined as *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001; ^#P < 0.05, ^##P < 0.01, ^###P < 0.001, ^####P < 0.0001 (* indicates significance between different groups and # indicates significance between different regions within the same group).