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. 2023 Mar 6;10(13):2206737. doi: 10.1002/advs.202206737

Figure 2.

Figure 2

RA‐treated tumor cells promote DCs‐mediated T cells activation. B16‐OVA cells were treated with DMSO or RA (5 µM) for 20 h, followed by coculturing with BMDCs and B3Z cells for an additional 24 h, then the activation of B3Z cells were measured by A) LacZ activity, B) secretion of IL‐2, and C) IFN‐γ, and D) surface expression of CD69. B16‐OVA cells were treated with DMSO or RA (5 µM) for 20 h, followed by coculturing with BMDCs and OT‐I cells for an additional 24 h, then OT‐I activation was examined by E) IL‐2 and F) TNF‐α production, G) surface expression of CD69, H) effector molecules GZMB, and I) IFN‐γ production. J–O) B16‐OVA cells were treated with DMSO or RA (5 µM) for 20 h, then cocultured with BMDCs for an additional 24 h, after which surface expression of CD40, CD80, CD86, MHC‐I, MHC‐II, and MHC‐I‐SIINFEKL on CD11c+ BMDCs was determined by FACS. Data are shown as mean ± SEM of 3 independent experiments. A–O) ** p < 0.01, *** p < 0.001, unpaired Student's t‐test.