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. 2023 Feb 25;10(13):2205436. doi: 10.1002/advs.202205436

Figure 5.

Figure 5

Pten/Akt/mTOR pathway regulates hepatic Me1 expression. A) Hepatic Me1 expression was measured in Ptenflox/flox and PtenAlb/Alb mice subjected to I/R injury at different months old. n = 3 per group. B) Immunoblots of hepatic Pten, p‐Akt, Akt, p‐mTOR, mTOR, and Me1 were analyzed in mice with sham or I/R injury. C) Schematic diagram depicting the targets of bpV and 3BDO. D) Hepatic levels of Me1 mRNA were measured in I/R‐treated mice with bpV or 3BDO injection. n = 4, 5, 4, 5. E,F) Ratios of NADPH/NADP+ (E) and GSH/GSSG (F) were measured in the livers of I/R‐treated mice with bpV or 3BDO injection. n = 4, 5, 4, 5. G) Hepatic cysteine contents were measured in I/R‐treated mice with bpV or 3BDO injection. n = 4, 5, 4, 5. H,I) Hepatic Ptgs2 mRNA (H) and MDA levels (I) were measured in I/R‐treated mice with bpV or 3BDO injection. n = 4, 5, 4, 5. J–L) Serum levels of ALT (J), AST (K), and LDH (L) were measured in I/R‐treated mice with bpV or 3BDO injection. n = 4, 5, 4, 5. M) Representative H&E‐stained liver sections from indicated mice. Significance was calculated by one‐way ANOVA; *p < 0.05, **p < 0.01, n.s. = not significant.