Table 2.
List of 58 proteins differentially expressed in LN positive GBC. This includes 53 proteins specific to LN positive GBC (A) and 5 proteins showed opposite correlation in expression among the proteins common to both the stages (B). The detailed protein and peptide list is provided in Supplementary Table S1
| LN Negative GBC | LN Positive GBC | |||||||
|---|---|---|---|---|---|---|---|---|
| Gene Symbol | Protein Name | GBC1 | GBC2 | GBC3 | GBC4 | GBC5 | GBC6 | GBC7 |
| (A) | ||||||||
| A1BG | Alpha-1B-glycoprotein precursor | 1.296 | 0.538 | 0.51 | 0.447 | 0.581 | 0.883 | 0.734 |
| ADH1C | Alcohol dehydrogenase 1C | 0.809 | 4.096 | 1.362 | 0.486 | 0.656 | 0.627 | 0.448 |
| AGR2 | Anterior gradient protein 2 | 0.99 | 0.53 | 1.998 | 1.582 | 4.208 | 1.685 | 0.712 |
| ALDH18A1 | Delta-1-pyrroline-5-carboxylate synthase isoform X1 | 1.126 | 0.867 | 1.471 | 3.227 | 1.416 | 0.927 | 1.246 |
| ANP32A | Acidic leucine-rich nuclear phosphoprotein 32 family member A | 0.845 | 0.808 | 1.534 | 2.300 | 1.439 | 1.214 | 1.219 |
| ANXA10 | Annexin A10 isoform X1 | 0.886 | 1.017 | 1.623 | 4.130 | 1.087 | 1.283 | 0.889 |
| AOC3 | Membrane primary amine oxidase isoform X1 | 0.559 | 0.749 | 0.856 | 0.50 | 0.733 | 0.703 | 0.485 |
| APEX1 | DNA-(apurinic or apyrimidinic site) lyase | 1.372 | 0.688 | 1.223 | 2.057 | 1.462 | 1.311 | 1.311 |
| APOC3 | Apolipoprotein C-III precursor | 0.68 | 1.408 | 0.466 | 0.165 | 0.158 | 0.109 | 0.245 |
| CAPG | Macrophage-capping protein isoform X1 | 1.274 | 0.606 | 0.877 | 1.363 | 1.103 | 1.402 | 2.061 |
| CKMT1A | Creatine kinase U-type, mitochondrial isoform 1 precursor | 1.163 | 0.786 | 1.367 | 3.211 | 1.695 | 1.445 | 1.014 |
| COL1A2 | Collagen alpha-2(I) chain precurso | 0.677 | 0.654 | 0.623 | 0.520 | 0.486 | 0.563 | 0.621 |
| COX4I1 | Cytochrome c oxidase subunit 4 isoform 1, mitochondrial isoform X1 | 0.712 | 1.593 | 1.013 | 2.687 | 1.265 | 0.843 | 1.012 |
| COX5A | Cytochrome c oxidase subunit 5A, mitochondrial precursor | 0.652 | 1.782 | 1.321 | 2.817 | 1.397 | 0.894 | 1.006 |
| COX6B1 | Cytochrome c oxidase subunit 6B1 | 0.891 | 1.431 | 1.406 | 2.289 | 1.047 | 1.140 | 0.977 |
| DLST | Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial isoform 1 precursor | 0.756 | 1.553 | 1.66 | 3.701 | 2.754 | 0.988 | 1.215 |
| ECH1 | Delta(3,5)-Delta(2,4)-dienoyl-coa isomerase, mitochondrial isoform X1 | 0.934 | 1.796 | 1.434 | 1.135 | 2.459 | 0.993 | 0.957 |
| EEF1A1 | Elongation factor 1-alpha 1 | 0.665 | 1.741 | 1.307 | 2.511 | 1.817 | 0.666 | 3.143 |
| FABP5 | Fatty acid-binding protein 5 | 0.895 | 0.545 | 1.464 | 0.827 | 1.438 | 1.035 | 8.795 |
| GMDS | GDP-mannose 4,6 dehydratase isoform 1 | 1.033 | 0.937 | 1.398 | 3.061 | 0.816 | 0.937 | 0.853 |
| HADHB | Trifunctional enzyme subunit beta, mitochondrial isoform X1 | 0.988 | 1.565 | 1.457 | 2.118 | 1.514 | 0.995 | 1.093 |
| HNRNPA1 | Heterogeneous nuclear ribonucleoprotein A1 isoform X1 | 0.618 | 0.813 | 1.613 | 3.609 | 3.282 | 1.135 | 2.160 |
| HNRNPA2B1 | Heterogeneous nuclear ribonucleoproteins A2/B1 isoform X1 | 0.523 | 0.831 | 1.256 | 2.594 | 2.939 | 0.807 | 2.714 |
| HNRNPA3 | Heterogeneous nuclear ribonucleoprotein A3 isoform a | 0.699 | 0.659 | 1.356 | 1.376 | 3.393 | 0.672 | 2.360 |
| HNRNPK | Heterogeneous nuclear ribonucleoprotein K isoform X1 | 0.91 | 0.816 | 1.922 | 2.541 | 2.434 | 1.183 | 2.334 |
| HSP90AA1 | Heat shock protein HSP 90-alpha isoform 1 | 0.743 | 0.976 | 1.611 | 1.744 | 2.088 | 1.139 | 1.574 |
| HSP90AB1 | Heat shock protein HSP 90-beta isoform X1 | 0.802 | 0.858 | 1.903 | 2.421 | 1.800 | 1.345 | 2.728 |
| HSPA8 | Heat shock cognate 71 kda protein isoform X1 | 0.712 | 1.162 | 1.067 | 1.588 | 1.588 | 1.726 | 2.270 |
| HSPA9 | Stress-70 protein, mitochondrial precursor | 0.796 | 1.664 | 1.656 | 3.183 | 2.044 | 1.037 | 2.357 |
| IDH2 | Isocitrate dehydrogenase [NADP], mitochondrial isoform 1 precursor | 1.011 | 1.722 | 1.231 | 2.148 | 1.310 | 0.744 | 1.181 |
| KRT1 | Keratin, type II cytoskeletal 1 | 1.418 | 0.585 | 0.606 | 0.473 | 0.768 | 0.611 | 0.897 |
| KRT17 | Keratin, type I cytoskeletal 17 | 1.088 | 0.765 | 1.091 | 0.827 | 1.556 | 4.911 | 7.180 |
| KRT19 | Keratin, type I cytoskeletal 19 | 1.002 | 0.749 | 1.788 | 7.241 | 3.853 | 2.390 | 0.930 |
| KRT6A | Keratin, type II cytoskeletal 6A | 1.252 | 0.732 | 1.397 | 1.202 | 0.888 | 1.352 | 2.212 |
| KRT7 | Keratin, type II cytoskeletal 7 | 0.878 | 0.72 | 0.963 | 2.651 | 1.944 | 2.497 | 0.820 |
| LASP1 | LIM and SH3 domain protein 1 isoform a | 1.531 | 1.045 | 1.107 | 2.971 | 1.467 | 0.881 | 1.703 |
| LRPPRC | Leucine-rich PPR motif-containing protein, mitochondrial precursor | 1.312 | 0.659 | 1.544 | 3.315 | 2.019 | 0.905 | 1.217 |
| MDH2 | Malate dehydrogenase, mitochondrial isoform 1 precursor | 0.797 | 1.519 | 1.651 | 3.556 | 2.404 | 1.465 | 1.694 |
| NPM1 | Nucleophosmin isoform 1 | 0.72 | 1.224 | 1.131 | 3.362 | 0.835 | 1.725 | 2.930 |
| PGD | 6-phosphogluconate dehydrogenase, decarboxylating isoform 1 | 1.811 | 0.967 | 1.04 | 1.270 | 1.084 | 1.132 | 2.052 |
| PKM | Pyruvate kinase PKM isoform c | 0.9 | 0.599 | 1.345 | 2.065 | 1.333 | 1.416 | 1.940 |
| PPIA | Peptidyl-prolyl cis–trans isomerase A isoform 1 | 0.733 | 0.908 | 1.3 | 1.778 | 1.747 | 1.446 | 2.435 |
| PPIB | Peptidyl-prolyl cis–trans isomerase B precursor | 0.746 | 1.261 | 1.31 | 1.577 | 1.066 | 1.279 | 2.299 |
| RPS28 | 40S ribosomal protein S28 | 0.82 | 1.712 | 1.642 | 2.046 | 1.871 | 0.681 | 2.114 |
| SERPINH1 | Serpin H1 isoform X1 | 0.988 | 0.881 | 1.269 | 1.046 | 1.450 | 1.061 | 2.587 |
| SLC25A3 | Phosphate carrier protein, mitochondrial isoform a precursor | 0.975 | 1.608 | 2.098 | 2.371 | 1.585 | 0.895 | 1.314 |
| SLC25A6 | ADP/ATP translocase 3 | 1.247 | 1.422 | 1.776 | 2.021 | 1.701 | 0.950 | 1.430 |
| SRI | Sorcin isoform A | 0.868 | 0.753 | 1.011 | 0.828 | 2.380 | 2.394 | 0.941 |
| TAGLN2 | Transgelin-2 isoform | 0.566 | 0.535 | 0.634 | 0.965 | 1.624 | 1.629 | 2.082 |
| TUFM | Elongation factor Tu, mitochondrial isoform 1 precursor | 0.975 | 0.828 | 1.582 | 3.457 | 1.009 | 0.803 | 1.609 |
| TYMP | Thymidine phosphorylase isoform 1 precursor | 1.97 | 1.138 | 1.024 | 0.905 | 1.045 | 1.252 | 2.441 |
| VCAN | Versican core protein isoform 1 precursor | 0.626 | 0.725 | 0.895 | 0.690 | 1.059 | 3.567 | 0.807 |
| VIM | Vimentin | 0.686 | 0.522 | 0.685 | 0.387 | 0.863 | 1.376 | 1.479 |
| (B) | ||||||||
| ADH4 | Alcohol dehydrogenase 4 isoform 1 | 0.50 | 9.87 | 2.15 | 0.49 | 0.50 | 0.47 | 0.43 |
| ANXA2 | Annexin A2 isoform 1 | 0.54 | 0.47 | 0.58 | 1.51 | 1.29 | 2.94 | 2.09 |
| ANXA5 | Annexin A5 | 0.76 | 0.40 | 0.52 | 1.60 | 1.17 | 2.75 | 1.15 |
| RDH16 | Retinol dehydrogenase 16 isoform 1 | 0.53 | 3.57 | 3.54 | 0.68 | 0.49 | 0.39 | 0.50 |
| UGT2B7 | UDP-glucuronosyltransferase 2B7 isoform 1 precursor | 0.49 | 3.62 | 1.34 | 0.68 | 0.49 | 0.38 | 0.37 |
The proteins marked in bold are DEPs with two-fold change, adjusted p value < 0.05 and identified with ≥ 2 unique peptides. DEPs- Differentially expressed proteins