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. 2023 Apr 14;9(4):e15482. doi: 10.1016/j.heliyon.2023.e15482

Table 4.

Different immunological-based methods for the identification of various foodborne pathogens in food samples.

Assay name Pathogen Food sample Detection time Detection limit References
ELISA Escherichia coli O157: H7 Vegetables 3 h 1 × 104 CFU/mL [59]
Salmonella typhimurium Milk 10 min 1.25 × 106 CFU/mL [291]
Cronobacter sakazakii Fruits 4 h 1 × 104 CFU/mL [292]
Lateral flow immunoassay Listeria monocytogenes Raw food 20 min 1 × 104 CFU/mL [293]
Shigella boydii Bread, milk, jelly 5–10 min 1 × 106 CFU/mL [294]
Immunofluorescence Assay Escherichia coli O157: H7 Meat 1 h 1.2 × 103 CFU/mL [295]
Immunomagnetic Separation Salmonella spp. Poultry 2 h 1 × 102 CFU/mL [296]
Latex agglutination assay Campylobacter spp. Milk, poultry, and meat 48 h 2.18 to 10.17 log10 CFU/mL [297]
Immunodiffusion assays Salmonella spp. Dairy product 14 h 1 × 105 CFU/mL [69]
Immunochromatographic assay Vibrio parahaemolyticus Sea food 4.5 h 1.58 × 102 CFU/mL [298]
Immunoglobulin G assay Staphylococcus aureus Meat 70 min 3.1 × 102 CFU/mL [71]
GLISA Bacillus cereus Spices and herbs NA 1.6–2.0 × 103 CFU/mL [72]

ELISA: enzyme-linked immunosorbent assay, GLISA: gold labeled immunosorbent assay.