Table 4.
Different immunological-based methods for the identification of various foodborne pathogens in food samples.
| Assay name | Pathogen | Food sample | Detection time | Detection limit | References |
|---|---|---|---|---|---|
| ELISA | Escherichia coli O157: H7 | Vegetables | 3 h | 1 × 104 CFU/mL | [59] |
| Salmonella typhimurium | Milk | 10 min | 1.25 × 106 CFU/mL | [291] | |
| Cronobacter sakazakii | Fruits | 4 h | 1 × 104 CFU/mL | [292] | |
| Lateral flow immunoassay | Listeria monocytogenes | Raw food | 20 min | 1 × 104 CFU/mL | [293] |
| Shigella boydii | Bread, milk, jelly | 5–10 min | 1 × 106 CFU/mL | [294] | |
| Immunofluorescence Assay | Escherichia coli O157: H7 | Meat | 1 h | 1.2 × 103 CFU/mL | [295] |
| Immunomagnetic Separation | Salmonella spp. | Poultry | 2 h | 1 × 102 CFU/mL | [296] |
| Latex agglutination assay | Campylobacter spp. | Milk, poultry, and meat | 48 h | 2.18 to 10.17 log10 CFU/mL | [297] |
| Immunodiffusion assays | Salmonella spp. | Dairy product | 14 h | 1 × 105 CFU/mL | [69] |
| Immunochromatographic assay | Vibrio parahaemolyticus | Sea food | 4.5 h | 1.58 × 102 CFU/mL | [298] |
| Immunoglobulin G assay | Staphylococcus aureus | Meat | 70 min | 3.1 × 102 CFU/mL | [71] |
| GLISA | Bacillus cereus | Spices and herbs | NA | 1.6–2.0 × 103 CFU/mL | [72] |
ELISA: enzyme-linked immunosorbent assay, GLISA: gold labeled immunosorbent assay.