Figure 4.

House dust mite sublingual allergen immunotherapy (AIT) does not alter polyinosinic:polycytidylic acid (poly(I:C))–induced epithelial type 2 cytokines in patients with allergic asthma. Human bronchial epithelial cells from patients with allergic asthma were stimulated with 10 μg/ml poly(I:C) for 24 hours. Cytokines were measured in cell-free supernatants using multiplex ELISA. (A–C) Protein release (absolute values) of IL-4 (A), IL-13 (B), and IL-10 (C). (D–F) Relative changes of protein release (end of treatment [Week 24] [EOT] − baseline [BL]) of IL-4 (D), IL-13 (E), and IL-10 (F). Relative change (Δ) values are presented as median with interquartile range; placebo (n = 17) and AIT (n = 14–17) for protein release. P values <0.05 were considered to indicate significant differences.