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. 2023 May 5;13:7352. doi: 10.1038/s41598-023-34069-z

Table 8.

The sequences (5′–3′) of primers and probes for IL1B three htSNPs examined.

rs Number Primers Probes
rs1143633
 Forward primer CTACTGGTGTTGTCATCAGAC
 Reverse primer AGCTTTTTTGCTGTGAGTCCC
 Common probe -P-CCTCGCCTCACGAGGCCTGCCCTTC-HEX-
 Discriminating probe G TTTTCCTCCAAGAAATCAAATTTTGCCG
 Discriminating probe A TCCTCCAAGAAATCAAATTTTGCCA
rs3136558
 Forward primer TCGCCATGTTGGCCAGGCTGG
 Reverse primer GTGGTCCTGCCAGGAACCATG
 Common probe -P-CTTTAGAAGCTCGGGATTCTTTCAATTT-HEX-
 Discriminating probe C TTTTCACGCCTGGCCCAGAGAGGGATGAC
 Discriminating probe T TTTTTTTCACGCCTGGCCCAGAGAGGGATGAT
rs1143630
 Forward primer GAATAGCCTGTAAGGTGTCAG
 Reverse primer GGATGCAGTAAGCCAAGATTG
 Common probe -P-CTTAACCTCCTTGAGCTTCAGAGAGTTTTTTTTT-FAM-
 Discriminating probe A TTTTTTTTTTTTCCTGCTGTGTGCCCTTGAGTACACA
 Discriminating probe C TTTTTTTTTTTTTTTCCTGCTGTGTGCCCTTGAGTACACC