FIG. 2.

Nucleotide sequence of the insert of pACM204 and deduced amino acid sequences for two ORFs corresponding to ampR and ampC. The standard single-letter code for amino acids is used; the stop codon is marked with an asterisk. Putative ribosomal binding sites (rbs), promoters (−10, −35), and selected restriction sites are indicated. Two in-frame alternate start codons for ampC are double underlined. The putative active-site serine is boxed; amino acids in boldface are characteristic motifs of serine β-lactamases. The predicted cleavage point for the signal peptide is marked with an arrowhead.