FIGURE 2:
E-cad dissembles at cell–cell junction and assembles at the basal side of RasV12 cells during dissemination. (a–e) Representative images of cells stained with anti-Arm antibody (gray). Genotypes are shown. Top images representing the basal section of the cells, projections of two to four very basal z-stacks. Middle images show cross-sections capturing cell–cell junction regions of the cells. In the orthogonal views, the basal side of cells is positioned upward. Yellow arrowheads indicate Arm signals detected at cell–cell junctions, and red arrowheads indicate Arm signals detected as distinct puncta at the basal side of RasV12 cells. Scale bar, 10 µm. (f) Quantification of Arm signal at cell junctions normalized to cytosolic Arm signal. From left to right, P = 1.43 × 10–13 (esgts - RasV12), P = 1.50 × 10–13 (esgts - RasV12, E-cad-iJF02769), P = 1.44 × 10–13 (esgts - RasV12, E-cad-iHMS0693). n = 30 cells from 10 midguts per genotype. (g) Total percentage of cells with Arm basal puncta per gut. From left to right, P = 1.64 × 10–5 (RasV12 - RasV12, E-cad-iJF02769) and P = 8.94 × 10–6 (RasV12 - RasV12, E-cad-iHMS0693); n = 12 (esgts), n = 11 (esgts>RasV12), n = 8 (esgts>RasV12, E-cad-iJF02769), and n = 9 (esgts>RasV12, E-cad-iHMS0693) midguts. Mean ± SEM are shown with individual data points. Statistical analysis was performed using one-way ANOVA with post-hoc Tukey HSD. Asterisks indicate statistical significance (*P < 0.05).