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. 2023 Jan 9;32(10):1647–1659. doi: 10.1093/hmg/ddad004

Figure 3.

Figure 3

Administration of L7-Slc9a6-GFP AAV modestly, but significantly increases glycosylated NHE6 and Slc9a6 mRNA. (A) Western blotting of cerebellar extracts with Abcam antibody shows that the glycosylated NHE6 band is reduced in carriers, and fully reduced in shakers administered the control AAV. A faint band is seen in shaker rats treated with L7-Slc9a6-GFP AAV with ICV injection at 5 weeks of age. (B) Repeat of (A) with ThermoFisher antibody confirms lack of the glycosylated NHE6 band in shakers + L7-GFP AAV and presence in shakers + L7-Slc9a6-GFP AAV. (C) Quantified results from (A). L7-Slc9a6-GFP AAV significantly restores NHE6 expression in shaker rats compared with those administered the control AAV. (D) Slc9a6 mRNA expression is evaluated through qRT-PCR. Slc9a6 is reduced in shaker rats compared with wt or carrier rats, whereas administering L7-Slc9a6-GFP AAV increases expression.