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. 2023 May 5;13:7376. doi: 10.1038/s41598-023-32927-4

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© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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(A) HC69.5 cells were treated with 100 µg/ml of poly IC and different concentrations of CBD or THC. After 24 h of drug treatment, the XTT-activated reagent was added. Cells treated with LPS were used as positive control. (B) Similarly, cells without poly IC were treated with different concentrations of CBD or THC. After 24 h of drug treatments, the XTT-activated reagent was added, and absorbance was measured after 4 h. Results are presented as a percentage of control, untreated HC69.5 cells. GraphPad Prism 5 software was used to plot and conduct statistical analysis. Normality distribution was checked by Kolmogorov–Smirnov test, while ANOVA and Dunns calculated statistical significance as a post-hoc test. Experiments were performed at least three times in replicates.