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. 2023 May 5;10(4):e200116. doi: 10.1212/NXI.0000000000200116

Figure 6. Effect of the Removal of Jo-1 Antibodies From Jo-1 Antibody Myositis on Complement-Dependent Cytotoxicity in TSM15 Cells.

Figure 6

(A) Western blotting demonstrated the reduction of Jo-1 antibodies. (B) Representative immunostaining to detect complement-dependent cytotoxicity in TSM15 cells before and after the reduction of Jo-1 antibodies. Calcein-AM stains live cells green while EthD-III stains dead cells red. Scale bar, 100 μm. (C) The removal of Jo-1 antibodies from 2 IgGs (500 µg/mL) with Jo-1 antibody myositis significantly decreased the complement-dependent cytotoxicity in TSM15 cells. Data are shown as the mean ± SEM of 6 independent experiments. Statistical significance was determined by an unpaired 2-tailed t-test. Jo-1 Ab removal (−), Jo-1-IgG before the reduction of Jo-1 autoantibodies; Jo-1 Ab (+), Jo-1-IgG after the reduction of Jo-1 autoantibodies.