Figure 3. Protein production and growth are diffusion limited processes.
a. Effective expression rate (kexp) of PADH2-mCherry decreases under osmotic stress in hog1Δ cells. b. kexp was fitted by a power-law function of the effective diffusion of 40nm-GEMs. c. Growth rate is proportional to protein production rate for hog1Δ cells under osmotic stress and WT cells under GIP. In all data, values are mean ± standard error of the mean, over N ≥ 3 independent biological replicates.