Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Oct 31;5:25152564221136388. doi: 10.1177/25152564221136388

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage).

PMC Copyright notice

Figure 1. — Vps13 is important for efficient autophagy. (A) Protein extracts were collected from wild-type (YLY085) and vps13Δ (YLY086) cell cultures in YPD grown to mid-log phase (SD-N 0 h) and 2 h after nitrogen starvation (SD-N 2 h). Western blot was performed with anti-YFP and anti-Pgk1 antibodies or antisera. (B) Quantitative analysis of the relative free GFP level. Free GFP levels were normalized to Pgk1 protein level then the relative free GFP levels were normalized to wild-type cells which were set to 1.0. (C) Protein samples were collected from the cell culture of wild-type (WLY176) and vps13Δ (WXY206) cells grown to mid-log phase in YPD (SD-N 0 h) and 4 h after nitrogen starvation (SD-N 4 h). Autophagy activity was measured with the Pho8Δ60 assay. Pho8Δ60 activities were normalized to wild-type cells after 4-h nitrogen starvation. (D) Protein extracts were collected from wild-type (YLY113, vac8Δ) and vps13Δ (YLY114, vac8Δ) cell cultures grown in YPD to OD₆₀₀ = 0.4–0.5 (SD-N 0 h) and 30 and 60 min after nitrogen starvation. Western blots were probed with anti-Ape1 and anti-Dpm1 antibodies. (E) Quantitative analysis of the relative mature Ape1 to total Ape1 (Ape1 + prApe1) ratio. The ratio of wild-type cells after 60-min starvation was set to 1 and other samples were normalized accordingly. In the quantitative analysis, the error bar represents the standard deviation (SD) of three independent experiments. Two-tailed t test was used for statistical significance. **p < 0.01, ***p < 0.001.