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. 2023 Apr 20;12:e83209. doi: 10.7554/eLife.83209

Figure 1. YAP expression is spatiotemporally regulated.

(A). YAP was expressed in both cushion mesenchyme and endothelium (triangles) at E11.5. (B). YAP is intensively expressed in VICs (white circles) at E14.5 but seldom detected in VECs (dashed arrows). (C). YAP expression remains in VICs on the tip regions and in VECs on the fibrosa side at E17.5 (solid arrows), while disappears in VECs on the ventricularis or ventricularis (dashed arrows) (D) Intensity ratios of nuclear vs. cytoplasmic YAP expressions in VECs and VICs of AV and SL valves at different stages. Data are presented by mean ± SEM, n=6 sections from three embryos, *p<0.05, two-way ANOVA tests. OFTC, outflow tract cushion; AVC, atrioventricular cushion; I, inferior cushion; S, superior cushion; D, distal cushion; P, proximal cushion; LA, left atrium; LV, left ventricle; RV, right ventricle; IVS, interventricular septum; V, ventricularis; A, atrialis; F, fibrosa.

Figure 1—source data 1. YAP activation measurement for Figure 1D.

Figure 1.

Figure 1—figure supplement 1. YAP expression in chick hearts during embryonic development.

Figure 1—figure supplement 1.

LA, left atrium; RA, right atrium; LV, left ventricle; RV, right ventricle; V, ventricularis; A, atrialis; F, fibrosa. Scale bar: 100 μm.
Figure 1—figure supplement 2. qPCR analysis of (A) mouse and (B).

Figure 1—figure supplement 2.

chick embryonic valves at different developmental stages, measures YAP transcriptional activity and the effect of Hippo pathway on YAP activities. Mouse data were normalized to E11.5 data, chick data were normalized to HH25 data. n>10 isolated valves. *p<0.05, two-way ANOVA tests.