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. 2023 Apr 20;12:e83209. doi: 10.7554/eLife.83209

Figure 4. Activation of YAP promoted cell proliferation and inhibited valve elongation.

(A-B). Cushion explants were cultured under U+PY-60 (unloaded +YAP activator), TS +PY-60 (tensile stress +YAP activator), TS (tensile stress) conditions for 24 hr. (C). After 24-hr-culture, explants were stained for YAP (green) and proliferation marker pHH3 (red, arrows), or (D). YAP (green) and endothelial cell-cell junction VE-Cadherin (red). (E). Circularity of explants cultured under different stress conditions, which describes how close a valve is to a perfect sphere. (F). Stiffness of cushion explants cultured with YAP activator and inhibitor, which was measured by micropipette aspiration measurement. (G). Percentages of cells expressing pHH3 under different culture conditions. (H). Average intensities of VE-Cad expression under different culture conditions, the intensities are normalized to maximum intensity. Data are presented by mean ± SEM, n=15 explant valves from eight embryos, *p<0.05, two-tailed student t-tests.

Figure 4—source data 1. Data used to generate Figure 4E-H.

Figure 4.

Figure 4—figure supplement 1. Stiffness of cushion explants.

Figure 4—figure supplement 1.

(A). Pressure-stretch curves of cushion explants treated with YAP inhibitor and activator, as well as the control. n≥10 explants. (B). Micropipette aspiration measurements, dash lines indicate tissue displacements within the tips.