REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit Human Serum Albumin Polyclonal antibody	Abcam	Cat# ab2406; RRID: AB_303048
Goat ZO-1 Polyclonal Antibody	Thermo Fisher Scientific	Cat# PA5-19090; RRID: AB_10988668
Mouse CYP3A4 Monoclonal Antibody (Clone 3H8)	Thermo Fisher Scientific	Cat# MA5-17064; RRID: AB_2538535
Mouse Anti-MRP2 Monoclonal Antibody (Clone M2 III-6)	Abcam	Cat# ab3373; RRID: AB_303751
Alexa Fluor 488-AffiniPure Donkey Anti-Goat IgG (H+L) (min X Ck,GP,Sy Hms,Hrs,Hu,Ms,Rb,Rat Sr Prot)	Jackson ImmunoResearch Labs	Cat# 705-545-147; RRID: AB_2336933
Cy3-AffiniPure Donkey Anti-Rabbit IgG (H+L) (min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Hu,Ms,Rat,Shp Sr Prot)	Jackson ImmunoResearch Labs	Cat# 711-165-152; RRID: AB_2307443
Alexa Fluor® 647 AffiniPure Donkey Anti-Mouse IgG (H+L)	Jackson ImmunoResearch Labs	Cat# 715-605-150; RRID: AB_2340862
Biological samples
Cryopreserved primary human hepatocytes	Gibco	Cat# HMCPSQ; lot HU8339-A
Cryopreserved primary human hepatocytes	Lonza	Cat# HUCPG; lot HUM180201A
Cryopreserved primary human hepatocytes	Gibco	Cat# HMCPMS; lot HU8287
XTreme 200 POOL HUMAN LIVER MICROSOMES	Tebubio	Cat#H2630; Lot nr: 171008
Chemicals, peptides, and recombinant proteins
Neratinib	Selleckchem	Cat# S2150
Nilotinib	Selleckchem	Cat# S1033
Ketoconazole	Selleckchem	Cat# S1353
Amiodarone HCl	Selleckchem	Cat# S1979
Fluticasone propionate	Selleckchem	Cat# S1992
Methadone stock solution	This paper	n/a
Critical commercial assays
CellTiter-Glo® 3D Cell Viability Assay	Promega	Cat# G9681
P450-Glo™ Assay with Luciferin-IPA	Promega	Cat# V9001
P450-Glo™ CYP1A2 Assay	Promega	Cat# V8771
HCS LipidTOX™ Red neutral lipid stain	Thermo Fisher Scientific	Cat# H34476
Human Albumin ELISA Quantitation Set	Bethyl Laboratories	Cat# E88-129
Experimental models: Cell lines
Induced pluripotent stem cell WTC-11	Coriell Institute for Medical Research	RRID:CVCL_Y803
Induced pluripotent stem cell WTSIi013-A	Wellcome Trust Sanger Institute	RRID:CVCL_AE60
Induced pluripotent stem cell WTSIi028-A	Wellcome Trust Sanger Institute	RRID:CVCL_AI14
Software and algorithms
Fiji	Schneider et al.7	https://imagej.nih.gov/ij/
PEAKS X+ software version 10.5	Bioinformatics Solutions	https://www.bioinfor.com/peaks-studio/
GraphPad PRISM 7	GraphPad Software Inc.
All original code has been deposited at GitHub repository and is publicly available via Zenodo: https://doi.org/10.5281/zenodo.7628912	Developed in-house during the project	https://doi.org/10.5281/zenodo.7628912