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. 2023 May 5;13:7321. doi: 10.1038/s41598-023-34143-6

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of PSO on recovery of lung tissue from MTX-induced lung injury (H&E). Control and PSO groups showed normal histological structure of lung alveoli, bronchi and interstitial tissue. MTX group showed severe interstitial pneumonia with abundant edema and heavy mononuclear inflammatory cells infiltration, the higher magnification (20x) showed that the bronchiolar lumen was occluded with mucous exudate and inflammatory cells with desquamated epithelial cells. MTX + PSO group showed marked reduction of inflammatory reaction in the lung parenchyma. Chart represents the lung score injury of different groups. Values are expressed as means ± SE. @ significant from Con, # significant from MTX. Significant difference is considered at p < 0.05.