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. 2023 May 5;14:2605. doi: 10.1038/s41467-023-38221-1

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — RNA-Seq (normalized read count) and RT-qPCR (mRNA fold change) were used to represent the changes in translational efficiency per polysome fraction and gene target. The Y axis represents the read counts normalized by DESeq2’s median of ratios. The X axis shows polysome fraction distributed per experimental condition in two panels, basal changes (left panel in each graph) and changes to mediate drug resistance (right panel in each graph). Drug-treated (+), not treated (–). a The S-adenosylmethionine synthetase (MAT, LmjF.30.3500) typically upregulated in Sb^III-resistant parasites. P value (MS): 0.84, P value (LP): 0.11, P value (HP): 0.009 (*). b The Kinetoplastid membrane protein-11 (KMP-11, LmjF.35.2210) is typically downregulated in Sb^III-resistant parasites. P value (MS): 0.76, P value (LP): 0.50, P value (HP): 0.094. c The ATP-binding cassette protein subfamily H (ABC-H, LmjF.11.0040) is typically upregulated in Sb^III-resistant parasites. P value (MS): 0.69, P value (LP): 0.06, P value (HP): 1.33 × 10⁻⁰⁵(*). d δ-amastin (LmjF.31.0450) a surface protein with an unknown role in Sb^III-resistance. P value (MS): 0.63. P value (LP): 0.02 (*). P value (HP): 7.53 × 10⁻¹⁰ (*). a–d Two-sided Wald test. Multiple comparison corrected by the Benjamini–Hochberg Procedure. Adjusted P value for drug challenge condition (right side of the figure). n: three biologically independent replicates. The shift in translational efficiency was also detected by RT-qPCR analysis. e Expression of ABC-H as linear fold change distributed per polysome fraction and experimental condition after qPCR analysis. f Expression of δ-amastin as linear fold change distributed per polysome fraction and experimental condition after RT-qPCR analysis. e, f n: two independent biological replicates with three technical replicates. Data are presented as mean values +/– SD. Threshold of significant fold change ≥1.5 (horizontal red line) for RT-qPCR. Monosomes (MS), light polysomes (LP), and heavy polysomes (HP). The raw data of (a–f) are available in Source Data.