Fig. 7. Resistant parasites modulate the synthesis of proteins involved in interconnected processes to combat Sb^III challenge.

DTTs detected in antimony-resistant parasites growing under drug challenge were submitted to GO analysis and protein–protein interaction network. a Significantly enriched cellular component GO categories including cytoplasmic vesicle, flagellum, and vacuole. b Significantly enriched molecular function GO categories including antioxidant modulators and ion-binding proteins. c Significantly enriched biological process GO categories supporting the activation of antioxidant response. a–c Upregulated DTTs (pink-red bars and nodes). Downregulated DTTs (blue bars). GO categories significantly enriched were filtered by FDR ≤ 0.05 using Benjamini–Hochberg method. d STRING protein–protein interaction network suggesting that proteins involved in glutathione/trypanothione (two molecules of glutathione joined by spermidine) metabolism are translated with high efficiency. Ascorbate peroxidase (APX, LmjF.34.0070), tryparedoxin peroxidase (TDPX, LmjF.36.0800), methionine sulfoxide reductase (METK1, LmjF.07.1140, LmjF.30.3500). e DTTs manually grouped based on the annotated gene description. The raw data of (a–e) are available in Source Data.